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Diagnostic pathway

Use our interactive diagnostic pathway to discover the various diagnostic methods for IFDs

Several diagnostic approaches are available for IFDs

Conventional testing, which includes:

  • Culture
  • Direct microscopy
  • Histopathology

Non-conventional testing, which includes:

  • 1,3-β-D-glucan (BDG) biomarker detection
  • Mannan detection
  • Cryptococcal antigen testing
  • Galactomannan biomarker detection
  • Polymerase chain reaction (PCR), including pan-fungal and strain-specific PCR screening assays

Navigate your way through the diagnostic pathway of a patient with clinical signs of infection


  1. Bassetti M et al. Clin Infect Dis. 2021;72:S121–S127.
  2. De Pauw B et al. Clin Infect Dis. 2008;46:1813–1821.
  3. Verweij PE et al. Intensive Care Med. 2020;46:1524–1535.
  4. Koehler P et al. Lancet Infect Dis. 2021;21:e149–e162.
  5. Cuenca-Estrella M et al. J Antimicrob Chemother. 2011;66:i15–24.
  6. Terrero-Salcedo D and Powers-Fletcher MV. J Clin Microbiol. 2020;58:e01487-19.
  7. Donnelly JP et al. Clin Infect Dis. 2020;71:1367–1376.
  8. Arvanitis M et al. Clin Microbiol Rev. 2014;27:490–526.
  9. Jenks JD et al. Clin Infect Dis. 2021;73:e1737–44.
  10. Mikulska M et al. Crit Care. 2010;14:R222.
  11. Kidd SE et al. Front Microbiol. 2019;10:2903.
  12. Faculty of Public Health, Health Knowledge. Available at: Accessed July 2023.

SciA-AFN-2300021 | July 2023